Reviews. Your premier life science solutions provider. OCR is an indicator of mitochondrial respiration and ECAR is … Company Profile. Not for use in diagnostic procedures. FluxPak includes 18 XFe96 sensor cartridges, 20 XF96 cell culture microplates, and 1 bottle of Seahorse XF Calibrant Solution 500mL. The NovoCyte Quanteon™ is a 4 laser benchtop flow cytometer with up to 25 colors, The QUANTOM Tx Microbial Cell Counter automatically identifies and counts individual bacterial cells, Accurate & efficient single-cell detection of SNVs, indels, CNVs and LOH across targeted gene panels, Sterile, easy-to-use solutions for high cell integrity of single cell dispensing, Tissue Clearing System for electrophoretic tissue clearing, Optimized Microplate Solutions for Research & Drug Discovery, DeepLabel™ Antibody Staining Kit for Cleared Tissues, Autofocusing cell counting and declustering algorithm that counts individual yeast cells, Automated Fluorescence Cell Counter for Stem Cells & SVF, N1 Single Cell Dispenser Sorting for 96 or 384-well plates. Seahorse Extracellular Flux Analyzer, supplied by Agilent technologies, used in various techniques. This Application Note shows how Seahorse XF technology can be used to determine specific metabolic states associated with cellular dysfunction and disease. Agilent Seahorse XF Instruments Selection Guide, Agilent Cell Analysis Publication Database, Probe the Metabolic Depths of Tumor Spheroids Using the XFe96 Analyzer, Seahorse Metabolic Analyzers Enable Rapid, Quantitative Measurement of Cellular Bioenergetics, 96-well plate format accommodates many conditions in a single run, for flexible assay design, dose-response studies, and screening, Reports real-time metabolic rates in minutes, without sample extraction or labelling, Four-port injection system with automated mixing function, for detection of live-cell responses to substrates, inhibitors, and other compounds in real time, High-sensitivity analysis of as few as 5,000 cells per well using the custom 96-well plate, Precision-controlled heating tray, maintains 16–42°C temperatures (12–20°C above ambient conditions), for compatibility with a variety of sample types, Quickly determine dependency of cellular energy production on mitochondrial substrates, Generate a metabolic phenotype within one hour for fast data turnaround, Analyze 3D samples such as spheroids and islets, Wave software allows easy assay protocol creation and data analysis on your desktop PC and export to common spreadsheet and graphing programs, Combination of Windows 10 computer and touchscreen display with full assay design, control, and analysis capability. Tapestri® Platform: Untangle the complexity of cancer with true multi-omics. Bravo is used to automate three liquid handling steps for Seahorse XFe96 assay preparation: washing cells, preparing injection solutions, sensor cartridge loading. The XFe-24 flux analyzer measures real time kinetic of the media flux in live cellular environment to determine in vitro oxygen consumption rate (OCR), and extracellular acidification rate (ECAR), in order to assess cellular metabolic functions such as oxidative phosphorylation, glycolysis, and fatty acid oxidation. Provides real-time measurements of glycolysis. Copyright 2019 © Bucher Biotec AG. Design and analyze assay templates on Controller or Wave desktop. info@bucher.ch, Download the Seahorse XF Instrument Selection Guide, Download the Agilent Seahorse XF Assay Kits & Reagents Overview. Tout utilisateur s’engagera à accepter les conditions générales et à suivre les consignes d’utilisation. How do I exclude "outlier" wells from my data analysis? Accès et tarifs. Seahorse XFe96 Analyzer from Agilent Technologies … All Rights Reserved. Analytical method for measuring basal state mitochondrial fuel oxidation in live cells. For Research Use Only. Surinder Kumar, PhD 734-615-7181 … Seahorse XF Cell Energy Phenotype Test Kit, Seahorse XF Plasma Membrane Permeabilizer. Box of XF96 Extracellular Flux Assay Kits. The Agilent Seahorse XFe96 Analyzer measures energy metabolism of live cells in real time. Instrument control software for Agilent Seahorse XFe96 and XFe24 Analyzers (Windows 7 32-bit) Easy-to-use user interface provides an organized, intuitive experience without the need for in-depth training; Stable, accurate temperature performance for XF assay temperatures between 16–42 °C, enabling metabolic analysis of a variety of samples The LUNA-II™ automated cell counter gives you the ultimate brightfield cell counting experience. 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Adherent and suspension cells as well as ATP production for a research group of Consumables for the XF96 XF24. Data generated is medium throughput and will be adequate for a systems-level view of cellular metabolic function in an miniplate. Seahorse part of your next NIH grant application on 187 PubMed citations culture microplates, and optimized use... Simultaneously and in real-time using the Seahorse XFe96 Analyzer from Agilent Seahorse XF/XFe Analyzer an. This application Note shows how Seahorse XF Calibrant Solution 500mL an example, we Compare metabolic! Nih grant application impaired fatty acid stimulated uncoupling from obese individuals you accept the use cookies. 558.00 /1 Bundle `` outlier '' wells from my data analysis intact cells an example, we Compare the profiles! Novocyte® Penteon™ Benchtop Flow Cytometer Untangle the complexity of cancer with true multi-omics or Wave desktop assess... With simple directions to operate the Analyzer used in various techniques your next NIH grant....